In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium Anabaena variabilis

In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium Anabaena variabilis: I. Some Characteristics of Fatty Acid Synthesis.

In vitro fatty acid synthesis was examined in crude cell extracts, soluble fractions, and 80% (NH(4))(2)SO(4) fractions from Anabaena variabilis M3. Fatty acid synthesis was absolutely dependent upon acyl carrier protein and required NADPH and NADH. Moreover, fatty acid synthesis and elongation occurred in the cytoplasm of the cell. The major fatty acid products were palmitic acid (16:0) and st...

In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium, Anabaena Variabilis: II. Acyl Transfer and Complex Lipid Formation.

In vitro fatty acid transfer to form complex lipids was observed in crude cell extracts of Anabaena variabilis using [1-(14)C]palmitoyl-acyl carrier protein, [1-(14)C]stearoyl-acyl carrier protein, and [1-(14)C]oleoyl-acyl carrier protein substrates. The data indicated that there was a rapid transfer of the fatty acids into the complex lipids. The greatest amount of radioactivity was observed i...

Bioenergetics Studies of the Cyanobacterium Anabaena variabilis

A cell-free system exhibiting both photophosphorylation (P /2e = 1) and oxidative phosphoryla­ tion (P/O up to 0 .8 ) is described for the cyanobacterium Anabaena variabilis. NADH and N A D PH were found to be equally effective as electron donors for oxidative phosphorylation. Low concentrations of U H D B T , an inhibitor of the cytochrome blc complex of mitochondria and chloroplasts. were fou...

RNA processing of nitrogenase transcripts in the cyanobacterium Anabaena variabilis.

Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied by using a lacZ reporter. Despite evidence for a transcription start site just upstream of nifH1 and vnfH, promoter fragments that included ...

The effect of ethanol on fatty acid metabolism; stimulation of hepatic fatty acid synthesis in vitro.